![]() We also found that the percentage of mosquitoes in which NI-2B viruses were dominant was significantly higher than that in which NI-1 viruses were dominant on day 7 but not at later time points. At 7 dpi, NI-2B viruses displayed a significantly higher replicative index in legs and salivary glands however, this advantage was lost by 14 and 21 dpi. Results consistently showed a significant replicative advantage of NI-2B over NI-1 viruses early after infection in vitro, and in mosquitoes, NI-2B viruses attained a higher replicative index than NI-1 isolates 3 to 7 days postinfection (dpi). ![]() In coinfection experiments, several different pairs of NI-1 and NI-2B clinical isolates were used to infect Aag2 cells or blood-fed A. aegypti mosquitoes reared from eggs collected in Managua. Here, we describe our studies of the replicative ability of NI-1 and NI-2B viruses in an A. We previously reported a clade replacement of the DENV-2 Asian-American genotype NI-1 clade by the NI-2B clade in Managua, Nicaragua. albopictus mosquitoes, causing up to 390 million DENV infections worldwide each year. ![]() The four dengue virus (DENV) serotypes (DENV serotype 1 to DENV-4) are transmitted by Aedes aegypti and A. ![]()
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